2023 Fiscal Year Final Research Report
Structure and function analysis of a novel Lewis blood group carbohydrate antigen utilization factor from human gut bacteria.
| Project/Area Number |
21K15025
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 43020:Structural biochemistry-related
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| Research Institution | Meiji University (2023)
The University of Tokyo (2021-2022) |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | ルイス抗原 / 腸内細菌 / 構造解析 / 糖質加水分解酵素 / 糖鎖 |
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| Outline of Final Research Achievements |
Human gut bacteria contribute to human health by producing short-chain fatty acids such as acetate and butyrate. Novel glycoside hydrolase and binding protein were discovered from butyrate-producing Roseburia sp.. Roseburia sp. possesses the HMO utilization pathways and can utilize Lewis a/b antigen from human milk oligosaccharides in breast milk in the intestines of weaned children. In this study, we aimed to understand the function of the enzyme and the uptake-binding protein at the molecular level and to clarify the structures in the human gut microbiota formation during weaning. The novel enzyme from Roseburia sp. was expressed, purified, and crystallized, and crystals were obtained and X-ray diffraction data were obtained.
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| Free Research Field |
構造解析
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| Academic Significance and Societal Importance of the Research Achievements |
ルイスa/b抗原はヒトミルクオリゴ糖などに存在する重要な糖鎖構造である。腸内細菌由来の新規なルイスa/b抗原認識部位の特異的な配列が明らかにされることで、分子レベルでの機能理解を深めることが可能となる点で学術的意義がある。また、保存された活性残基やアミノ酸配列から他の微生物ゲノム情報に基づいて同様の活性を有しているか明らかにすることが可能になり、腸内細菌形成メカニズムの解明に寄与する知見が得られる点において社会的意義がある。
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