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2023 Fiscal Year Final Research Report

Structure-guided engineering of a biodegradable plastic synthase for tailor-made polymer production

Research Project

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Project/Area Number 21K15028
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 43020:Structural biochemistry-related
Research InstitutionNara Institute of Science and Technology

Principal Investigator

CHEK MIN FEY  奈良先端科学技術大学院大学, 先端科学技術研究科, 助教 (60834455)

Project Period (FY) 2021-04-01 – 2024-03-31
KeywordsBiodegradable plastic / Polyhydroxyalkanoate / Enzyme engineering
Outline of Final Research Achievements

PHA synthase (PhaC) is the key enzyme in the biosynthesis of polyhydroxyalkanoate (PHA), a biodegradable plastic that offers a promising alternative to petrochemical plastics. In this study, the crystal structure of PhaC was determined, and important regions for catalysis were identified. Specific positions in the egress tunnel of PhaC were mutated, and the mutated enzymes were purified and their enzymatic activities measured. Additionally, the exact catalytic mechanism of PhaC was elucidated through biochemical and enzymatic analyses. The outcomes of this study provide an information for future engineering of PhaC to broaden its substrate specificity.

Free Research Field

Structural biology, Enzymology

Academic Significance and Societal Importance of the Research Achievements

This study reveals the structure-function relationship of the biodegradable plastic-synthesizing enzyme PHA synthase (PhaC). It is utmost urgent to improve the efficiency and substrate range of PHA production, catalysed by PhaC, in order to reduce the production costs of the biodegradable plastics.

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Published: 2025-01-30  

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