2022 Fiscal Year Final Research Report
Acquisition and validation of anti-bRIL shark VNAR antibodies for the development of a versatile structural analysis tool
| Project/Area Number |
21K15030
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 43020:Structural biochemistry-related
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| Research Institution | Meiji University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2023-03-31
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| Keywords | VNAR / bRIL / anti-body |
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| Outline of Final Research Achievements |
This study aimed to obtain a shark antibody against apocytochrome b562RIL (bRIL) which is frequently used for stabilizing membrane-proteins. Human antibodies grab antigens by using two domains, whereas shark antibodies pierce them by its single domain. Because of this difference, we aimed to apply shark antibodies to structural analysis. Since the titer of the anti-bRIL antibody in blood was gradually increased, immunizing with bRIL protein against sharks, we attempted to obtain anti-bRIL antibodies by phage display method. However, we couldn't obtain the antibody. It was found that strengths and weaknesses of shark antibodies depending on the shape of the antigen protein in this study.
Translated with www.DeepL.com/Translator (free version)
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| Free Research Field |
食品化学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究ではアポシトクロムb562RIL(bRIL)に対するサメ抗体の取得を目指しましたが、取得には至りませんでした。同時並行していた別のタンパク質でのサメ抗体取得には成功しましたので、手法の問題とは考えにくく、bRILが有している安定なヘリックス構造という特徴が、サメ抗体の特徴である突き刺さるように認識するという性質とは合わなかったのではないかと推察されました。
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