Specificities of cellular uptake of primary cilium-derived extracellular vesicles for intercellular communication

2021 Fiscal Year Research-status Report

• Project/Area Number: 21K15088
• Research Institution: Hiroshima University
• Principal Investigator: IJAZ FARYAL 広島大学, 医系科学研究科(医), 助教 (80845595)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: primary cilium / Extracellular vesicles

Outline of Annual Research Achievements

Primary cilium protrudes from the cell surface like an antenna into the extracellular environment surrounding the cell and detect and transduce sensory stimuli to the cell body. Here we show that primary cilium also effects cell signaling and processes through primary cilium-dependent biofactors. The preliminary data results show that the cell migration and proliferation was enhanced in biofactors treated cells in a primary cilium dependent manner.

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

The project is going rather smoothly because we have already obtained preliminary data for the role of primary cilium-dependent bioactive factors.

Strategy for Future Research Activity

Task1. Confirmation of effects of primary cilium-dependent biofactors: (a) Preparation of knock-out cells deficient in primary cilium (b) Check the effects of primary cilium-dependent biofactors in different cell lines.
Task2. Evaluation of secretion profile: Identification of biofactors and signaling pathways they are involved in.
Task3. Invitro and invivo models for bioassays of primary cilium-dependent biofactors and potential for cell therapeutics.

Causes of Carryover

Due to the personal sick leave some aspects of the research project could not be done. The remaining amount will be used for making transgenic cell lines models and purchasing cell lines.