2023 Fiscal Year Annual Research Report
Specificities of cellular uptake of primary cilium-derived extracellular vesicles for intercellular communication
| Project/Area Number |
21K15088
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| Research Institution | Hiroshima University |
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Principal Investigator |
IJAZ FARYAL 広島大学, 医系科学研究科(医), 助教 (80845595)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | Primary Cilium / Ciliopathies / Humoral Factors |
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| Outline of Annual Research Achievements |
We examined the influence of extracellular bioactive factors derived from the culture supernatant of wildtype NIH/3T3 cells on the migratory capacities of primary cilium-deficient NIH/3T3-Kif3a-KO cells after wounding. The factors derived from the culture supernatant of wildtype NIH/3T3 cells increased the rate of cell migration in target cells compared to the factors derived from the culture supernatant of primary cilium-deficient NIH/3T3-Kif3a-KO cells. Additionally, through omics analysis of the supernatants, we identified a specific molecular constituent exhibiting elevated concentrations in the culture supernatant of primary cilium-deficient cells. These findings suggest that fibroblasts regulate the cellular migration in primary cilium-deficient target cells by humoral bioactive factors in a primary cilium-dependent manner.
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