Specificities of cellular uptake of primary cilium-derived extracellular vesicles for intercellular communication

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K15088
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 44010:Cell biology-related
• Research Institution: Hiroshima University
• Principal Investigator: Ijaz Faryal 広島大学, 医系科学研究科(医), 助教 (80845595)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: primary CIlium

Outline of Final Research Achievements

In this study, we examined the influence of extracellular bioactive factors derived from the culture supernatant of wildtype NIH/3T3 cells on the migratory and proliferative capacities of primary cilium-deficient NIH/3T3-Kif3a-KO cells after wounding. The factors derived from the culture supernatant of wildtype NIH/3T3 cells increased the rate of cell migration/proliferation in target cells compared to the factors derived from the culture supernatant of primary cilium-deficient NIH/3T3-Kif3a-KO cells. Additionally, through omics analysis of the supernatants, we identified a specific molecular constituent exhibiting elevated concentrations in the culture supernatant of primary cilium-deficient cells. These findings suggest that fibroblasts regulate the cellular migration/ proliferation process in primary cilium-deficient target cells by humoral bioactive factors in a primary cilium-dependent manner.

Free Research Field

Cell Biology

Academic Significance and Societal Importance of the Research Achievements

繊毛症における繊毛欠損細胞の細胞内因性決定因子の解明は大きく前進しましたが、繊毛欠損細胞からの細胞外因性因子がこれらの病態に及ぼす影響については、まだ比較的未開拓の領域です。この研究は、繊毛症が初めて細胞外因性の観点から研究されたという意味で重要です。これは、将来、繊毛症を治療するための治療法を見つける道を開くものでもあります。