2022 Fiscal Year Final Research Report
Protein import into endosymbionts becoming organelles
| Project/Area Number |
21K15094
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 44010:Cell biology-related
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
Husnik Filip 沖縄科学技術大学院大学, 進化・細胞・共生の生物学ユニット, 准教授 (30886130)
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| Project Period (FY) |
2021-04-01 – 2023-03-31
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| Keywords | symbiosis / metabolomics / proteomics / FIB-SEM / uCT / SBF-SEM / genomics / FISH |
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| Outline of Final Research Achievements |
In the frame of the project, I have focused on host-symbiont integration in a unique bacterium-within-bacterium-within-eukaryote symbiosis found in mealybugs. Via the combination of omics (genomics, transcriptomics, proteomics, and metabolomics) with 3D imaging (uCT, confocal FISH imaging, SBF-SEM, FIB-SEM), we managed to understand which genes, proteins, and metabolites are localized in the system and how they drive the tripartite interaction. Integrating data from all these advanced methods is unprecedented for a single symbiotic species and allows us to draw strong conclusions about host-symbiont integration (e.g. cell division coordination and protein import) in highly interdependent, organelle-like, symbioses.
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| Free Research Field |
evolutionary cell biology
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| Academic Significance and Societal Importance of the Research Achievements |
Understanding how highly integrated symbioses function is essential(and relates to human health) since one organelle in our cells, the mitochondrion, has originated from a symbiotic bacterium.
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