2022 Fiscal Year Research-status Report
Differential dopamine dynamics of DMS and DLS projecting SNc neurons during reversal learning
| Project/Area Number |
21K15184
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | Dopamine / Basal Ganglia / Goal-directed behavior / dLight / Striatum |
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| Outline of Annual Research Achievements |
Elucidation of the changes of the phasic activity of the DMS and DLS projecting SNc neurons, identified direct and indirect pathway striatal neurons, and local striatum dopamine release in rats performing a probabilistic reward-based task. We used TH-Cre, Drd2-Cre and Tac-1-Cre transgenic rats for dopaminergic neurons and striatal projecting neurons identification. For the investigation of dopamine release in dorsal striatum, we express dLight in DMS and DLS and monitored the fluorescence using fiber photometry.
Elucidation of the differential role of intratelencephalic (IT) and corticofugal (CF) DMS corticostriatal OFC and mPFC in rats performing a probabilistic reward-based task. We use a viral vector approach for the identification of the different DMS corticostriatal neurons.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The article titled 'Reward expectation enhances action-related activity of nigral dopaminergic and two striatal output pathways', where we examined how neuronal activity of substantia nigra pars compacta (SNc) and dorsal striatum, and local striatal dopamine release, depend on recent average rewards in rats performing a reward-based choice task, is currently under revision for publication in Communication Biology.
We currently are performing experiments for the identification od the different population of DMS projecting OFC and mPFC corticostriatal neurons and their role in goal-directed behavior.
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| Strategy for Future Research Activity |
1. Evaluation of SNc neuronal activity and dLight signal relation to action-outcome contingency change.
2. Simultaneous evaluation of local dopamine release in striatum (dLight) and striatal neurons activity (RCaMP) using fiber photometry.
3.Evaluation of DMS projecting OFC and mPFC corticostriatal neurons relation to monitoring and updating goal-directed behavior and action-outcome contingency change.
4. Performing optogenetical manipulation experiments (stimulation using channelrhodopsin; inhibition using Achearhodopsin) in DMS projecting OFC and mPFC corticostriatal neurons.
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| Causes of Carryover |
We intend to acquire some custom made instrument (manipulators), which requires time to manufacture. In addition to the try and error phase of such instruments, there was a delay in the purchase.
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