2022 Fiscal Year Research-status Report
Molecular mechanisms of postnatal amygdalar neurogenesis
| Project/Area Number |
21K15187
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
DAUN KENNY 滋賀医科大学, 医学部, 特任助教 (40896510)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | Postnatal Amygdala / Neurogenesis / Early-life stress / Lineage tracing |
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| Outline of Annual Research Achievements |
We postulated that the environmental stimuli during postnatal development might alter the proliferation, differentiation, and survival of newborn cells in the amygdala. However, the identity of the cell subtypes and their origins remains to be determined. Therefore, by identifying the cell-specific lineage affected by environmental stimuli in the postnatal amygdala, we will be able to clarify the functional identities of newborn cells and whether they contribute to adult processing later in adulthood. Last year, we injected the GFP-expressing lentiviruses into the right lateral ventricle of postnatal day 0 neonates, followed by MSD. The GFP+ cells were isolated, and the integration sites of the lentivirus were identified. The stereological analysis was performed, and data was obtained.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
There have been minor delays in certain aspects of the experiments, but overall, the research is progressing at a reasonable pace. Last year, I added single-cell RNA sequencing to the originally planned experiments using an additional budget. The individual cells were isolated and prepared, and the samples were sent for sequencing.
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| Strategy for Future Research Activity |
We will extract a set of unique genes obtained from the single-cell RNA sequencing datasets and determine the expression patterns of the newborn cells. We will also plan to construct GFP and tetracycline-inducible diphtheria toxin A-expressing lentivirus to ablate postnatal neurogenesis and analyze the function of neurons at different developmental stages. Finally, we will provide insight into the origins, cell subtypes, and differentiation capacity of newborn cells by identifying their specific cell lineage in the postnatal amygdala.
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