2021 Fiscal Year Research-status Report
Discovery and characterization of a pressure sensation and transduction (PREST) mechanism in the presynaptic terminal in the CNS.
| Project/Area Number |
21K15203
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| Research Institution | The University of Tokyo |
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Principal Investigator |
UCAR HASAN 東京大学, ニューロインテリジェンス国際研究機構, 特任助教 (50748423)
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| Project Period (FY) |
2021-04-01 – 2026-03-31
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| Keywords | Release probabilty / Optogenetics / iGluSnFr / Presynaptic terminal / FRET / Spine enlargement / Mechanosensation |
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| Outline of Annual Research Achievements |
The following article was published in Nature.
Mechanical actions of dendritic-spine enlargement on presynaptic exocytosis. Ucar et al., Nature, 600 (December 23, 2021), pp. 686-689.
With this study we announced the mechanical transmission and the PREST mechanism in the synapse which was a milestone in the neuroscience field.
I showed that enlarging spines pushed the presynaptic terminals, and this enhanced its activity by causing an increase in the synaptic vesicle docking onto plasma membrane and an increase in the evoked glutamate release.
I also showed that fine and transient (1 min) pushing of a presynaptic terminal by a glass pipette or application of osmotic pressure (by 20 mM sucrose) were sufficient to mimic the effects of spine enlargement.
Now, I am exploring molecular machineries underlying PREST signaling.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
We have published our results in Nature.
We are exploring the molecular machineries underlying PREST mechanisms. We have promising preliminary data on some candidate machineries.
Regulation of F-actin via Arp2/3 complex seems to be important for PREST signaling. Also instead of actin scaffold, Arp2/3 activity for F-actin dynamics could be more important.
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| Strategy for Future Research Activity |
I am planning to explore the interaction of F-actin dynamics with the synaptic vesicle (SV) mobilization.
I have observations that the SV clusters are mobilized upon mechanical stimulation and I want to explore the role of F-actin in this process.
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Research Products
(4 results)
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[Presentation] Mechanosensation and PREST in the presynaptic terminals2022
Author(s)
Hasan Ucar, Satoshi Watanabe, Jun Noguchi, Sho Yagishita, Yuichiro Morimoto, Noriko Takahashi, Haruo Kasai
Organizer
The 45th Annual Meeting of the Japan Neuroscience Society. The 65th Annual Meeting of the Japanese Society for Neurochemistry The 32nd Annual Conference of the Japanese Neural Network Society
Int'l Joint Research / Invited
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