2022 Fiscal Year Final Research Report
Screening for crude drug extracts that regulate spike protein cleavage in SARS-CoV-2
| Project/Area Number |
21K15283
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 47050:Environmental and natural pharmaceutical resources-related
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| Research Institution | Josai University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2023-03-31
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| Keywords | 生薬 / リポジショニング / 漢方薬 / SARS-CoV-2 / furin |
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| Outline of Final Research Achievements |
The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contains a furin recognition site (FCS) for furin-like enzymes at the boundary of the S1/S2 subunits. The cleavage of the site is essential for S protein activation and virus entry. We screened the inhibitory effects of crude drugs on in vitro furin-like enzymatic activities and mRNA expression analysis. Out of 130 herbal medicine extracts, we identified herbal medicines and compounds such as Cnidii Monnieris Fructus and osthole.
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| Free Research Field |
生薬学、分子生物学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究ではSARS-CoV-2のスパイクタンパク質（Sタンパク質）のプロセシングを制御する生薬エキスの探索を行った．細胞内furin様活性はSARS-CoV-2 Sタンパク質のみならず，種々のウイルスの膜タンパク質のプロセシングに関与している．今回furin様活性に対する阻害効果のある生薬エキスや化合物を同定することができたことで、これら感染症に対する治療薬・予防薬開発に繋がることが期待される．
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