Regulation of CRISPR-Cas3 deletion length by protein obstacles

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K15361
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 48040:Medical biochemistry-related
• Research Institution: Kyoto University
• Principal Investigator: Kojima Yusuke 京都大学, iPS細胞研究所, 特定拠点助教 (90896416)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: ゲノム編集 / CRISPR-Cas3

Outline of Final Research Achievements

In this project, we aimed to regulate CRISPR-Cas3 deletion length by using protein obstacles, and also, to establish a method to analyze collisions between Cas3 and other proteins on the genome. As its achievements, we could obtain many useful insights into Cas3 deletion patterns although practical and easy Cas3 regulation methods have not been established within this period. We also could develop an improved method to detect protein-protein collisions on the genome. Then, eventually, we could publish one research paper and submit one patent containing insights obtained from this project.

Free Research Field

ゲノム編集

Academic Significance and Societal Importance of the Research Achievements

本課題を通じて、新規国産ゲノム編集技術であるCRISPR-Cas3について、細胞内の動態をより理解でき、欠損領域をどのように制御するかという課題に対して多くの知見を得ることができた。これら得られた知見は、将来的にCas3を有用なゲノム編集技術として確立し、特に遺伝子疾患などの難病をゲノム編集や再生医療の技術を用いた治療に応用する上で非常に意義のあることであったと考える。