Comprehensive analysis of micro RNA in colorectal submucosal invasive carcinoma

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K15406
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 49020:Human pathology-related
• Research Institution: Iwate Medical University
• Principal Investigator: Eizuka Makoto 岩手医科大学, 医学部, 助教 (90815937)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 大腸癌 / リンパ節転移 / micro RNA / messenger RNA

Outline of Final Research Achievements

RNA was extracted from paraffin for colorectal invasive carcinoma (18 lymph node metastases, 21 non-metastases), and comprehensive miRNA and mRNA analyses were performed. For comparison of miRNA expression variation between the lymph node metastasis and non-metastasis groups, miRNAs with FDR p<0.05 and delta fold change > absolute value of 2 were selected. The miRNAs and mRNAs that were significantly expressed in the lymph node metastasis group were examined by pathway analysis and found to be regressive: METAP1 and miR-4685-3p, and TGOLN2 and miR-6806-3p. These miRNA-mRNA combinations may play an important role in lymph node metastasis.

Free Research Field

消化管腫瘍

Academic Significance and Societal Importance of the Research Achievements

大腸癌のリンパ節転移群、非転移群においてmicro RNA (miRNA)およびmessenger RNA (mRNA)解析を行い、リンパ節転移群で多く発現するものを抽出しその回帰性を示す組み合わせを検討したところ2組のmRNA/mRNAの組み合わせを抽出した。これらの2組のmRNA/mRNAの組みあわせが大腸癌のリンパ節転移に重要な役割を担う可能性が示唆され、大腸癌の予後不良因子となる可能性がある。症例の蓄積、追加検討による妥当性の検討が肝要と考えられる。