Pathophysiological analysis of Brugada syndrome using iPS cell-derived right ventricular cardiomyocytes

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K16057
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53020:Cardiology-related
• Research Institution: Okayama University
• Principal Investigator: Saito Yukihiro 岡山大学, 大学病院, 助教 (20724454)
• Project Period (FY): 2021-04-01 – 2023-03-31
• Keywords: iPS細胞 / 右室心筋細胞 / 二次心臓領域

Outline of Final Research Achievements

We established a method to induce left ventricular and right ventricular cardiomyocytes separately from healthy human iPS cells and found that the properties of left and right ventricular cardiomyocytes were different. The paper is currently being submitted for publication. In addition, we generated iPS cells from skin fibroblasts derived from three patients with Brugada syndrome, and induced differentiation of left ventricular and right ventricular cardiomyocytes from the iPS cell lines. Although it took time to optimize the combination of cell seeding density and concentration of low molecular weight compounds for each cell line, we were able to induce cardiomyocytes corresponding to left ventricular and right ventricular cardiomyocytes from all cell lines. Since we were not able to perform specific analysis, we plan to analyze the cardiomyocytes in the future.

Free Research Field

循環器内科学

Academic Significance and Societal Importance of the Research Achievements

ヒトiPS細胞によって、患者自身の心筋細胞を研究に用いることができるようになった。一方で、疾患を生じる心臓の部位には特徴のある疾患については、特徴を示しうる心筋細胞をiPS細胞から誘導することが重要であるはずである。本研究において確立された、健常人および患者由来iPS細胞から左室および右室心筋細胞を別々に効率よく誘導する方法は、Brugada症候群、不整脈原性右室心筋症、肺高血圧症、先天性心疾患などの右室疾患に対する細胞モデル研究をサポートする意義のある成果である。