2021 Fiscal Year Research-status Report
Enhancer based fine-tuning of Runx family for the regulation of HSC division during bone marrow recovery
| Project/Area Number |
21K16272
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2023-03-31
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| Keywords | hematopoietic stem cell |
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| Outline of Annual Research Achievements |
Chemotherapy induced bone marrow stress and subsequent Hematopoietic stem cells (HSCs) regeneration has been extensively studied, and yet the precise mechanism regulating the HSC divisional fate is not completely resolved. Since the accessibility-changed regions between self-renewal and differentiation divisions significantly enriched Runx-binding motifs during bone marrow regeneration, my proposed study will look into the Runx-based epigenetic modification of HSCs during the course of bone marrow regeneration. In this year, I compared chromatin accessibility of Runx enhancers between self-renewal and differentiation types of HSCs, and identify candidate of Runx enhancer regions that affect its expression.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
I did not establish the protocol for CRISPR-based deletion of Runx enhancers. However, I can recently get a good results regarding this.
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| Strategy for Future Research Activity |
By using CRISPR-based deletion of Runx enhancers, I will examine the effect of Runx enhancer deletion on HSC functions in vitro (e.g. culture) as well as in vivo (e.g. transplantation).
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| Causes of Carryover |
Since I had long business trip, the amount that I used was lower than that I planned.
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