2022 Fiscal Year Annual Research Report
Enhancer based fine-tuning of Runx family for the regulation of HSC division during bone marrow recovery
| Project/Area Number |
21K16272
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2023-03-31
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| Keywords | hematopoietic stem cell |
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| Outline of Annual Research Achievements |
Chemotherapy induced bone marrow stress and subsequent Hematopoietic stem cells (HSCs) regeneration has been extensively studied, and yet the
precise mechanism regulating the HSC divisional fate is not completely resolved. Since the accessibility-changed regions between self-renewal
and differentiation divisions significantly enriched Runx-binding motifs during bone marrow regeneration, my proposed study will look into the
Runx-based epigenetic modification of HSCs during the course of bone marrow regeneration. In this year, I compared H3K27 acetylation of
Runx enhancers between self-renewal and differentiation types of HSCs by CUT&Tag system. In addition, Runx3, which is related to differentiation, was highly expresssed in EPCR Low HSCs, compared to EPCR High HSCs that is most primitive HSCs.
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