Analysis of bioactive compounds released by bacteria for early detection

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K16318
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 54030:Infectious disease medicine-related
• Research Institution: Osaka University
• Principal Investigator: Yamasaki Seiji 大阪大学, 高等共創研究院, 准教授 (70757301)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 耐性菌 / 感染症 / 排出ポンプ / 細菌 / 抗生物質 / 抗菌薬 / 薬剤耐性 / 多剤耐性

Outline of Final Research Achievements

This research found that current signals with specific shapes reflecting cell size and ion leakage were generated by potential differences in a micropore-based bridge circuit and identified three bacterial species by classifying signal shapes using machine learning.
In addition, we discovered several common mutations in the bacterial drug efflux pump, which is responsible for the efflux of antibiotics and bioactive compounds from bacteria, present in drug-resistant Pseudomonas aeruginosa. We were able to submit the paper as an achievement that will lead to the development of a rapid method for determining bacterial resistance.
Furthermore, by analyzing and classifying the gases generated from the culture medium using machine learning, we succeeded in identifying bacterial species. The number of bacterial species is currently increasing and further analysis is underway for practical use.

Free Research Field

細菌学

Academic Significance and Societal Importance of the Research Achievements

早期に菌種の同定および耐性菌の検出ができれば、不要な抗菌薬投与を減らすことができ、細菌感染症による死亡者数を減らせるだけでなく、新たな耐性菌出現の抑制、医療費の削減にも大きく貢献することができる。研究期間全体を通じて、当目的の達成に寄与する新デバイスの開発に向けた技術基盤を構築することができた。