2023 Fiscal Year Final Research Report
Mechanism of viral particle formation based on HIV-1 proteins and RNA
| Project/Area Number |
21K16324
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 54030:Infectious disease medicine-related
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | HIV-1 / NCINI耐性変異 / HIV-1 RNA / HIV-1 integrase / HIV-1 nucleocapsid |
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| Outline of Final Research Achievements |
Ribonucleotide proteins (RNPs) have been reported to egress the HIV core by inhibiting HIV integrase (IN) binding to HIV-1 RNA. In this study, we investigated the profile of drug-resistant HIV-1 including HIV-1 RNA and HIV-1 protein interactions to elucidate the mechanism of HIV-1 particle formation. The viral particle morphology, infectivity, and replication ability of HIV-1 clones with the drug-resistant amino acids were evaluated. The phase separation and interaction between HIV-1 protein and HIV-1 RNA of these HIV-1 clones were investigated.
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| Free Research Field |
感染症、HIV感染症、真菌感染症、薬剤開発
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、高次構造依存的なHIV-1 RNAおよびHIV-1 タンパク質の相互作用、HIV-1 タンパク質の相分離現象等のアプローチから、HIV-1粒子形成メカニズムを解明することを目的とした。本研究は、タンパク質とRNAの相互作用による高次構造形成に新しい知見をもたらし、その学術的意義は非常に高いと言える。さらに、本研究での解析結果は、新薬開発における新たな標的部位の発見や阻害様式のヒントとなり、COVID-19に代表されるウイルス感染症の治療薬開発等に応用可能な新知見となり得る。
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