Characterization of tissue-specific glycocalyx components

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K16586
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55060:Emergency medicine-related
• Research Institution: Gifu University
• Principal Investigator: Tachi Masahito 岐阜大学, 医学部附属病院, 助教 (70646504)
• Project Period (FY): 2021-04-01 – 2023-03-31
• Keywords: glycocalyx

Outline of Final Research Achievements

Staining with seven lectins (ConA, DBA, PNA, RCA1, SBA, UEA1, and WGA) was performed on brain, lung, kidney, heart, liver, small intestine, and colon tissue. The DBA lectin binding was due to its binding to N-acetylglucosamine, and the WGA lectin binding was due to its ability to bind to N-acetylglucosamine, Hyaluronan is highly specific to cerebral blood vessels.

Free Research Field

救急医学

Academic Significance and Societal Importance of the Research Achievements

医療への応用を考え、将来、ヒト組織や非侵襲的画像検査によって、簡易にグリコカリックスを可視化することにつながるためには、より簡易で、臓器ごとの毛細血管におけるグリコカリックスを適した結合レクチンを用いて、観察する。今あるレクチン染色を最適化、最大化し、将来、ヒト組織や非侵襲的画像検査によって、簡易にグリコカリックスを可視化することにつなげたい。