Molecular mechanisms of IL1b-induced proliferation of synovial mesenchymal stroll cells

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K16678
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Matsumura Etsuko 東京医科歯科大学, 大学院医歯学総合研究科, 非常勤講師 (30831854)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: MSC / IL1b / CD121a / Proliferation

Outline of Final Research Achievements

To efficiently provide regenerative therapy for articular cartilage using autologous synovial mesenchymal stem cells (MSCs), it is crucial to establish a protocol that ensures the safe and reliable supply of autologous cells for transplantation. However, due to inherent variability in the proliferative capacity of patient-derived cells, maintaining a consistently sufficient number of transplantable cells remains a challenge. Our research has demonstrated that IL-1β acts as a potent growth factor for MSC proliferation without compromising their differentiation capacity. However, the subset of IL-1β receptor-positive cells in MSCs is relatively low, approximately around 5%, suggesting the presence of unidentified IL-1β signaling pathways in MSCs. The aim of this study was to elucidate the molecular mechanisms underlying IL-1β-induced MSC proliferation and apply this knowledge to advance tissue regeneration therapies.

Free Research Field

整形外科学

Academic Significance and Societal Importance of the Research Achievements

本研究は、MSC特異的に存在するIL1b受容体を介したMSC増殖の新規分子メカニズムの解析を行うものであり、先行研究は存在しない。私たちのこれまでの研究成果から、MSCと炎症性細胞で異なるIL1bに対する細胞応答性が観察される原因として、異なる受容体アイソフォームの発現が考えられたが、このようなIL1b受容体の生理機能は今まで報告されておらず、本研究はIL-1bの新たなバイオロジーの開拓につながると考えている。