Elucidation of Cancer Genomic Pathology in the Natural History of Non-Muscle Invasive Bladder Cancer

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K16726
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56030:Urology-related
• Research Institution: University of Tsukuba
• Principal Investigator: Kawahara Takashi 筑波大学, 医学医療系, 講師 (50758919)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 筋層非浸潤性膀胱癌 / BCG

Outline of Final Research Achievements

Non-muscle invasive bladder cancer (NMIBC) often recurs and progresses even after BCG intravesical therapy. To understand the mechanisms behind this, it is crucial to conduct longitudinal analyses that track changes in the cancer genome pathology within the same patient over time. We have demonstrated that DNA and RNA of consistent quality can be extracted from formalin-fixed paraffin-embedded (FFPE) samples obtained through transurethral resection of bladder tumors, regardless of tumor size or timing of resection. This finding allows for comprehensive analyses using historical samples. We plan to report on the longitudinal, comprehensive analyses of these samples, to elucidate the temporal changes in cancer genome pathology in the same patients with NMIBC.

Free Research Field

膀胱癌

Academic Significance and Societal Importance of the Research Achievements

近年、ホルマリン固定パラフィン包埋（FFPE）検体からDNA、RNAを抽出する技術が向上したが、経尿道的膀胱腫瘍切除で採取された様々なサイズの検体からどの程度DNA、RNAが抽出できるか明らかとなっていなかった。本研究で経尿道的膀胱腫瘍切除で採取された過去の小さな検体であっても網羅的解析が可能であることが明らかとなり、筋層非浸潤性膀胱癌の同一患者におけるがんゲノム病理の変化の解明の一助となると考えられる。また本研究で行った縦断的な網羅的解析により筋層非浸潤性膀胱癌のBCG抵抗性や早期再発/早期進展の分子学的機序を明らかとなることが期待される。