2022 Fiscal Year Final Research Report
Effects of Epithelial-Mesenchymal Transition on Immunometabolism in the Ovarian Cancer Microenvironment
Project/Area Number |
21K16768
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 56040:Obstetrics and gynecology-related
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Research Institution | Kyoto University |
Principal Investigator |
TAKI MANA 京都大学, 医学研究科, 助教 (20898077)
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Project Period (FY) |
2021-04-01 – 2023-03-31
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Keywords | Ovarian cancer / Tumor microenvironment / MDSC / tumor immunity |
Outline of Final Research Achievements |
Using ovarian cancer as a model, we aimed to search for how epithelial-mesenchymal transition of tumor cells affects immune cell metabolism in the tumor microenvironment. As immune cells, we focused on PMN-MDSCs, the granulocyte lineage of bone marrow-derived immunosuppressed cells (MDSCs), which showed significant differences between Snail-expressing tumors and controls. We extracted PMN-MDSCs from mouse tumors using a cell sorter and searched for changes in gene expression levels by RNA-seq, and found that only genes involved in inflammatory immunity, such as IL-10, showed differences in expression. We focused on EP1, one of the prostaglandin receptors that showed the most difference, and are exploring the possibility that it is a receptor specific to PMN-MDSCs.
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Free Research Field |
婦人科腫瘍
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Academic Significance and Societal Importance of the Research Achievements |
卵巣癌の免疫治療は臨床試験で有用な結果を得られず、現在模索中である。今回卵巣癌の免疫抑制で重要な働きをきたす骨髄抑制免疫抑制細胞に関して探索を行った。今回の研究成果で骨髄抑制免疫抑制細胞の新たな受容体の存在の可能性を検討しえた。骨髄抑制免疫抑制細胞を抑制することが可能となれば、卵巣癌の治療がさらに有用になると考える。
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