NGS-based analysis of the true causative bacteria of gynecological infections as a basis for new therapeutic strategies.

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K16777
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56040:Obstetrics and gynecology-related
• Research Institution: Keio University
• Principal Investigator: NOGAMI Yuya 慶應義塾大学, 医学部(信濃町), 助教 (90574294)
• Project Period (FY): 2021-04-01 – 2023-03-31
• Keywords: 骨盤内膿瘍 / 菌叢解析 / リンパ嚢胞感染 / 侵襲性溶連菌感染症

Outline of Final Research Achievements

Fifty-six specimens were collected from 44 patients with gynecological infections. 42 specimens were submitted to 16SrRNA-based bacterial flora analysis using NGS. By disease, 24 specimens were from infected lymphocysts, 9 specimens were from pyometra, and 9 specimens were from intra-abdominal abscess. Due to the COVID19 pandemic, the number of patients did not reach the expected number in this period. We plan to analyze 30 cases in total and to report, as for infected lymphocysts. Blood culture specimens from patients with lymphedema-based cellulitis have been stored. Group B streptococci are the most common causative organisms, and we plan to investigate the characteristics of these organisms. The distribution of causative organisms in pyometra associated with cervical cancer has not been reported so far. We plan to add more cases and analyze them.

Free Research Field

婦人科感染症

Academic Significance and Societal Importance of the Research Achievements

NGSを含めた新規解析方法により、菌の同定などが可能になってきているが、婦人科感染症は、感染症領域での検討がいまだ乏しい。新規技術を用いた新しい知見の模索により、新しい予防、診断、治療戦略のシーズとなりえる可能性がある。また婦人科悪性腫瘍治療後の合併症として、生じる感染症は、症例数の多い専門施設でしか集積が難しい。本研究期間内に一定数の検体が収集、解析できたことは、大きな意義があり、今後の研究に活かされる。