2022 Fiscal Year Final Research Report
Elucidating the Role of Macrophages in the Development of Endometriosis and Exploring Therapeutic Targets
Project/Area Number |
21K16783
|
Research Category |
Grant-in-Aid for Early-Career Scientists
|
Allocation Type | Multi-year Fund |
Review Section |
Basic Section 56040:Obstetrics and gynecology-related
|
Research Institution | The University of Tokyo |
Principal Investigator |
MAKABE Tomoko 東京大学, 医学部附属病院, 助教 (50851086)
|
Project Period (FY) |
2021-04-01 – 2023-03-31
|
Keywords | 子宮内膜症 / 免疫異常 / 腹腔内環境 / NK細胞活性 |
Outline of Final Research Achievements |
Intra-peritoneal monocyte components (PFMC) were extracted from the peritoneal fluid of endometriosis and non-endometriosis patients (operated for benign ovarian cysts and uterine leiomyoma) who underwent laparoscopic surgery at our hospital. The distribution of immune cell fractions was confirmed using CyTOF, a mass cytometric technique, and an examination focusing on NK cells was conducted first. Differentiation and activation of NK cells and activation of metabolic enzymes were examined at the single cell level and are currently being analyzed. In the future, we plan to quantify metabolites in the NK cell fractions identified by CyTOF, which are characteristic of endometriosis patients, using metabolonic analysis to identify metabolic pathways that cause NK cell dysfunction in endometriosis patients and to explore new therapeutic strategies.
|
Free Research Field |
生殖医学
|
Academic Significance and Societal Importance of the Research Achievements |
生殖年齢の子宮内膜症患者における、病態の慢性化は、生活の質の低下のみならず不妊の原因となるとともに、癌化リスクの上昇も伴い、その病態解明や治療法の確立は喫緊の課題である。子宮内膜症の発生や進展には局所の免疫担当細胞が関与する。中でも、NK細胞は、子宮内膜症や子宮内膜症関連癌の病態に密接に関わっていると考えられ、NK細胞の活性化調整機構に着目することで子宮内膜症の病態解明のみならず治療法開発の糸口となる可能性がある。
|