16s rRNA microbiota in cervical cancer

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K16796
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56040:Obstetrics and gynecology-related
• Research Institution: Wakayama Medical University
• Principal Investigator: Kobayashi Aya 和歌山県立医科大学, 医学部, 博士研究員 (50596971)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 子宮頸癌 / マイクロバイオーム

Outline of Final Research Achievements

With the approval of our hospital's ethics committee, we conducted a study on FFPE tissue samples from 14 cases of cervical cancer. 16s rRNA microbiome analysis using extracted tumor DNA was performed, and it was possible to identify and analyze the composition ratio of the bacterial species that make up the tissue flora of all 14 cases. However, since this study was aimed at general clinical use, FFPE tissue samples were used. In this case, the paraffin part without tissue was analyzed as a control, and although significant bacteria could be identified by subtracting the resulting bacterial flora, stable results were not obtained. This was thought to be due to differences in the bacteria enclosed with each case and the lack of aseptic processing.

Free Research Field

産婦人科学関連

Academic Significance and Societal Importance of the Research Achievements

本研究を遂行することにより、今まで確立されていなかった、子宮頸癌の進行や治療抵抗性メカニズムと組織内の細菌層との関連についての見解が得られる可能性があり、臨床背景のみならず、腫瘍の遺伝子変異や微小環境も含めた包括的検討を行う点についても新規性がある。さらに子宮頸癌の放射線治療症例について検討することにより、口腔癌・喉頭癌・食道癌・大腸癌などの細菌叢解析が可能な癌についても応用できる可能性を秘めており、今後の癌治療において意義深い知見が得ることができると考えられ、本研究を開始した。