Comparative validation of acetylcholine receptor expression patterns in the nasal cavity among different species.

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K16850
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56050:Otorhinolaryngology-related
• Research Institution: Hyogo Prefectural Amagasaki General Medical Center
• Principal Investigator: Ishikawa Masaaki 兵庫県立尼崎総合医療センター(研究部), その他, 医師 (10813743)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: アセチルコリン受容体 / 慢性副鼻腔炎 / 気管支喘息

Outline of Final Research Achievements

We aimed to investigate the heterogeneity in gene expression patterns of acetylcholine (Ach) receptors in the nasal/sinonasal tissues in mice, rats, and human, and the influence of clinical background on the patterns. To achieve the aim, we collected nasal/sinonasal tissues from the three different species, and then performed transcriptome analyses. In human, tissues were obtained from non chronic rhinosinusitis (CRS) as the control, eosinophilic CRS (ECRS), and non ECRS.
As a result, in mice and rats, the analyses were impossible due to the insufficient volume of RNA seq. In human, CHRM1, an acetylcholine receptor, was found as one of differentially Expressed Genes in control vs. ECRS. Three of the four ECRS patients had comorbidity of asthma. In both ECRS and non-ECRS, all patients had postoperative recurrence with nasal polyps.

Free Research Field

耳鼻咽喉科

Academic Significance and Societal Importance of the Research Achievements

CHRM1は、気管支喘息の病態に関与している(Maeda et al., 2006)。術後再発を起こす難治性慢性副鼻腔炎(CRS)において、controlと比較した際に好酸球性CRSのみにこのCHRM1が発現変動遺伝子として認めた。そのため、CHRM1と好酸球の関連性が難治性CRSの病態解明に重要になるのではないかと考える。好酸球が2型免疫反応に関与/2型免疫反応を制御する因子として副交感神経から放出されるAchの重要性が報告されており(Bosmans et al., 2017)、この経路はコリン作動性抗炎症反応経路として知られている。この経路に焦点を当て、難治性CRSの病態解明に繋げたい。