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2022 Fiscal Year Research-status Report

Temperature-driven regulation system of hypermucoviscosity in carbapenem-resistant Klebsiella pneumoniae

Research Project

Project/Area Number 21K16947
Research InstitutionHiroshima University

Principal Investigator

LE NGUYEN・TRA・MI  広島大学, 医系科学研究科(歯), 助教 (20897904)

Project Period (FY) 2021-04-01 – 2024-03-31
KeywordsKlebsiella pneumoniae / hypervirulent / multidrug resistance / antimicrobial peptide / bacteriocin
Outline of Annual Research Achievements

Multidrug-resistant and hypervirulent K. pneumoniae has been continuously evolving, which causes severe clinical outcome and significantly challenges the available chemotherapeutic treatment for its infections.
K. pneumoniae produces several kinds of antimicrobial peptides known as bacteriocins, which have antibacterial activity against closely-related species. Bacteriocins are considered one of K. pneumoniae virulence factors. We identified bacteriocin genes in 180 K. pneumoniae species complex genomes, including 170 hypermucoviscous isolates, and investigated the antibacterial activity against 50 strains, including antimicrobial-resistant organisms, belonging to multiple species. This study has been accepted on April 11th and published on May 8th in Microbiology Spectrum:
Le MN, Nguyen TH, Trinh VM, Nguyen TP, Kawada-Matsuo M, Kayama S, Sugai M, Komatsuzawa H. Comprehensive Analysis of Bacteriocins Produced by the Hypermucoviscous Klebsiella pneumoniae Species Complex. Microbiol Spectr. 2023 May 8:e0086323. doi: 10.1128/spectrum.00863-23. Epub ahead of print. PMID: 37154746.

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

The first paper has been published in Microbial Genomics in 2022, entitled "Genomic epidemiology and temperature dependency of hypermucoviscous Klebsiella pneumoniae in Japan" (Le MN, Kayama S, Wyres KL, Yu L, Hisatsune J, Suzuki M, Yahara K, Terachi T, Sawa K, Takahashi S, Okuhara T, Kohama K, Holt KE, Mizutani T, Ohge H, Sugai M. Genomic epidemiology and temperature dependency of hypermucoviscous Klebsiella pneumoniae in Japan. Microb Genom. 2022 May;8(5):mgen000827. doi: 10.1099/mgen.0.000827. PMID: 35622495; PMCID: PMC9465067.)
The second paper has been published in Microbiology Spectrum in May 8th 2023, entitled "Comprehensive analysis of bacteriocins produced by the hypermucoviscous Klebsiella pneumoniae species complex" (Le MN, Nguyen TH, Trinh VM, Nguyen TP, Kawada-Matsuo M, Kayama S, Sugai M, Komatsuzawa H. Comprehensive Analysis of Bacteriocins Produced by the Hypermucoviscous Klebsiella pneumoniae Species Complex. Microbiol Spectr. 2023 May 8:e0086323. doi: 10.1128/spectrum.00863-23. Epub ahead of print. PMID: 37154746.)

Strategy for Future Research Activity

Regarding the hypermucoviscosity (HMV) phenotype, investigation of the novel gene(s) responsible for hypermucoviscosity is being carried out using the isolates with medium/high-HMV phenotype and negative for rmpA and rmpA2 genes.
Regarding the bacteriocin theme, we are investigating novel bacteriocins produced by K. pneumoniae to develop potential alternative treatments for infections caused by multidrug-resistant bacteria.

  • Research Products

    (11 results)

All 2023 2022 Other

All Int'l Joint Research (2 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (2 results) (of which Int'l Joint Research: 1 results) Remarks (5 results)

  • [Int'l Joint Research] London School(英国)

    • Country Name
      UNITED KINGDOM
    • Counterpart Institution
      London School
  • [Int'l Joint Research] Monash University(オーストリア)

    • Country Name
      AUSTRIA
    • Counterpart Institution
      Monash University
  • [Journal Article] Comprehensive Analysis of Bacteriocins Produced by the Hypermucoviscous Klebsiella pneumoniae Species Complex2023

    • Author(s)
      Le Mi Nguyen-Tra、Nguyen Thao Huu-Huong、Trinh Van Minh、Nguyen Tam Phuc-Bao、Kawada-Matsuo Miki、Kayama Shizuo、Sugai Motoyuki、Komatsuzawa Hitoshi
    • Journal Title

      Microbiology Spectrum

      Volume: 11 Pages: 00863-23

    • DOI

      10.1128/spectrum.00863-23

    • Peer Reviewed / Open Access
  • [Journal Article] Genomic epidemiology and temperature dependency of hypermucoviscous Klebsiella pneumoniae in Japan2022

    • Author(s)
      Le Mi Nguyen-Tra、Kayama Shizuo、Wyres Kelly L.、Yu Liansheng、Hisatsune Junzo、Suzuki Masato、Yahara Koji、Terachi Tsuneko、Sawa Kana、Takahashi Shin、Okuhara Toshihiko、Kohama Kunihiko、Holt Kathryn E.、Mizutani Tetsu、Ohge Hiroki、Sugai Motoyuki
    • Journal Title

      Microbial Genomics

      Volume: 8 Pages: 1

    • DOI

      10.1099/mgen.0.000827

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Comprehensive analysis of bacteriocins produced by Klebsiella pneumoniae complex2023

    • Author(s)
      Mi Nguyen-Tra Le
    • Organizer
      The 96th Annual Meeting of Japanese Society for Bacteriology
  • [Presentation] Temperature-dependent hypermucoviscosity and genomic population structure of Klebsiella pneumoniae2022

    • Author(s)
      Mi Nguyen-Tra Le
    • Organizer
      13th International Meeting on Microbial Epidemiological Markers (IMMEM XIII)
    • Int'l Joint Research
  • [Remarks] Google Scholar

    • URL

      https://scholar.google.com/citations?user=SDEAg9gAAAAJ&hl=en

  • [Remarks] ResearchGate

    • URL

      https://www.researchgate.net/profile/Mi-Le-7

  • [Remarks] ORCID

    • URL

      https://orcid.org/my-orcid?orcid=0000-0002-9394-6107

  • [Remarks] Web of Science

    • URL

      https://www.webofscience.com/wos/author/record/GLN-7600-2022

  • [Remarks] Scopus

    • URL

      https://www.scopus.com/authid/detail.uri?authorId=57215581166

URL: 

Published: 2023-12-25  

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