2022 Fiscal Year Research-status Report
An idea of regulatory science-based bone regeneration product for critical bone defect healing
| Project/Area Number |
21K16962
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | Regenerated Bone / Nanoindentation / Implant Therapy / Functional Bone / Micro-CT analysis |
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| Outline of Annual Research Achievements |
The jawbones from the mice alveolar bone defect models were examined by Micro-CT scanning and histological analysis to assess new bone formation in the defect area at 4 and 8 weeks after implantation. The results of 3D image analysis and Hematoxylin and Eosin staining demonstrated that implanted MCOB-3DPL constructs have successfully regenerated bone within bone defects and is significantly higher than the other groups at 8 weeks post-implantation. Nanoindentation analysis has shown that regenerated bone induced by MCOB-3DPL constructs has micromechanical properties comparable to the native bone.
The results of implant therapy by Micro-CT and histological analysis revealed that regenerated bone formed by MCOB-3DPL constructs possesses osseointegration ability.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
It has been shown previously that this type of growth can be achieved through a three-dimensional arrangement of osteogenic cells, such as immature osteoblasts, in a scaffold. In our present study, we describe the development of a three-dimensional cotton-like scaffold structure produced by novel electrospinning technology using 3DPL. This 3DPL scaffold complex is suitable for MCOB differentiation to regenerate large jawbone defects. Most of the results are as expected and favourable towards the MCOB-3DPLA group. However, in one of the control group, we used cytrans is also showing comparable results to MCOB-3DPLA group. So are trying to find out are any significant differences between the measuring parameters of new bone and functional property between these two groups.
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| Strategy for Future Research Activity |
Immunohistochemical analysis of the defect area for selected bone markers to evaluate active remodelling processes around the regenerated new bone will be investigated.
Histological and 3D imaging analysis will be performed after four weeks of implantation to evaluate the levels of osseointegration, and the implant survival rates.
Scaffolds designed for tissue engineering need to be biocompatible with the regenerating cells and provide a suitable environment for attachment, proliferation and differentiation. Viable cells within the scaffold are required for bone regeneration, whereby cells secrete extracellular matrix or growth factors to induce new bone formation. Therefore, a Live dead assay will be performed to assess the viability of MCOBs on the seeded 3DPL4 scaffolds.
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[Presentation] Development of Bone Regeneration Product using Immature osteoblasts and three-dimensional polymer scaffold.2022
Author(s)
Venkata Suresh Venkataiah, Shigeto Suzuki, Yoshio Yahata, Akira Kitagawa, Masahiko Inagaki, Mary M. Njuguna, Risako Nozawa, Yusuke Kakiuchi, Masato Nakano, Keisuke Handa, Masahiro Yamada, Hiroshi Egusa and Masahiro Saito
Organizer
The 2022 Autumn Annual Meeting of JSCD (154th) & JKDC academic conference
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[Presentation] Development of bone tissue engineering technology using immature osteoblasts and three-dimensional polymer scaffold2022
Author(s)
Venkata Suresh Venkataiah, Shigeto Suzuki, Yoshio Yahata, Akira Kitagawa, Masahiko Inagaki, Mary M. Njuguna, Risako Nozawa, Yusuke Kakiuchi, Masato Nakano, Keisuke Handa, Masahiro Yamada, Hiroshi Egusa and Masahiro Saito
Organizer
The International Oral Health Symposium 2022.
