Analysis of inhibitor effect on SASP related factor and of appotosis inducind effect on senecence cells of japanease butter bur extract.

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K16973
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57030:Conservative dentistry-related
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: Takada Ayuko 北海道医療大学, 歯学部, 助教 (70825968)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: フキ / NO / SASP

Outline of Final Research Achievements

Butterbur extract suppressed the generation of NO, a kind of oxidant that causes cellular senescence, when added to oral epithelial cells (Ca9-22) after LPS stimulation, and also suppressed the production of iNOS. The results showed that the phosphorylation of c-jun was inhibited by Butterbur extract. Furthermore, the inhibitory effect of JNK inhibitor on NO production was highest in Ca-22 cells. Butterbur extract suppresses LPS-stimulated NO production in oral epithelial cells by inhibiting c-jun phosphorylation, thereby causing suppression of iNOS production.

Free Research Field

生化学

Academic Significance and Societal Importance of the Research Achievements

細胞老化の原因となる酸化物質の一種であるNOの産生を抑えることで、細胞の老化を抑えることが可能となれば、細胞老化による個体老化の抑制にもつながる。本研究では、北海道に自生する身近な植物であるフキの葉からのエキスを利用することにより環境にも配慮している。今後の研究では細胞老化に関わるSASP関連因子を含めてより深く探究する価値がある。