Mechanism of crosstalk between periodontal ligament and bone linage cells in the presence of mechanical stress.

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K16984
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57030:Conservative dentistry-related
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Tsuchiya Yosuke 東京医科歯科大学, 東京医科歯科大学病院, 特任助教 (40882072)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 咬合性外傷 / RNAシーケンス解析 / 歯周炎 / 歯根膜細胞

Outline of Final Research Achievements

Occlusal trauma is an important risk factor for the exacerbation of periodontitis, however the regulatory mechanisms remain unclear. The purpose of this study was to elucidate the molecular mechanisms by performing RNA-sequencing of periodontal tissues of periodontitis model and occlusal trauma model in mice.Micro-CT analysis showed no bone resorption in Tra, however, significantly increased bone resorption in LiTra compared to Li. No bone resorption was observed in Tra in long-term evaluation. Principal component analysis showed that gene expression patterns were different in each group, especially in bone. RNA-seq results for bone from Li and LiTra showed 167 differential expressed genes (|FC|>2, q<0.1), and marked increase in expression of genes related to inflammation.

Free Research Field

歯周病学

Academic Significance and Societal Importance of the Research Achievements

咬合性外傷は歯周炎を悪化させる重要な修飾因子として知られている一方で、分子生物学的なメカニズムの検討は乏しい状況であった。本研究は歯と骨を繋ぐ歯根膜細胞に着目し、同細胞がメカニカルストレスと骨の応答の架け橋となり、骨吸収を制御している可能性は高く、歯根膜由来の分子が骨芽細胞、破骨細胞、さらには骨細胞に働きかける制御機構の解明の解明につながると考えている。さらには咬合性外傷により変化する遺伝子群が解明されることで歯周病進行リスクの評価につながることも期待できる。