Investigation of the interaction between dental biofilm and gingival epithelium with the use of in situ dental biofilm model

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K16990
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57030:Conservative dentistry-related
• Research Institution: Niigata University
• Principal Investigator: Sotozono Maki 新潟大学, 医歯学総合研究科, 助教 (00876675)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: デンタルバイオフィルム / 細菌叢解析 / 歯肉上皮細胞

Outline of Final Research Achievements

Dental biofilm is the main cause of dental caries and periodontitis. However, dental biofilm can contribute the homeostasis of gingival epithelium, promoting immune system of the host. In this study, the interaction between gingival epithelial model and experimental dental biofilm formed by in situ dental biofilm model was investigated from two aspects, host and dental biofilm. The result of this study showed that microbiome of dental biofilm can change and be affected by host epithelial cell and dental biofilm induce the increase of the thickness of epithelial model.

Free Research Field

歯科保存学

Academic Significance and Societal Importance of the Research Achievements

細菌叢のバランスの乱れ（dysbiosis）がう蝕や歯周病の進行を助長し、全身疾患の重篤化にも影響を与えると考えられている。本研究により、歯肉上皮がデンタルバイオフィルム細菌叢に影響を与えることが示唆されたことから、単にデンタルバイオフィルムを除去することが疾患の予防方法ではなく、宿主側にアプローチをすることで、新たな疾患予防の確立に寄与できる可能性がある。