development of a new direct pulp capping material targeted the factor that is involved in the odontoblastic differentiation

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K16995
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57030:Conservative dentistry-related
• Research Institution: Kyushu University
• Principal Investigator: Fujino Shoko 九州大学, 歯学研究院, 助教 (60883832)
• Project Period (FY): 2021-04-01 – 2023-03-31
• Keywords: 直接覆髄 / 歯髄幹細胞 / 象牙芽細胞分化 / ドーパミン

Outline of Final Research Achievements

Conventional direct pulp-capping materials sometimes induce bone-like dentin with poor sealing properties. Therefore, exploration of biomolecules that allow tight sealing by tubular reparative dentin is required. We recently reported that dopamine (DA) is involved in dentinogenesis. Hence, we investigated the effect of DA on odontoblastic differentiation of DPSCs and reparative dentin formation. DA stimulation promoted the odontoblastic differentiation of DPSC and induced tubular reparative dentin. These results suggest that DA produced by TH is involved in odontoblastic differentiation of DPSCs and has an inductive capacity for reparative dentin formation similar to primary dentin. This study may lead to the development of therapy to preserve vital pulp tissues.

Free Research Field

歯科保存

Academic Significance and Societal Importance of the Research Achievements

歯髄組織の保存は、将来的な歯の保存につながると考えられる。現在の歯科治療において、偶発的露髄が生じた際、歯髄を保存するために直接覆髄処置が行われる。現在臨床で頻用されている水酸化カルシウム製剤およびMTAセメントは、多孔性の骨様象牙質を形成する場合が多いため、十分な封鎖ができず、感染を起こして抜髄となるケースは少なくない。そこで、本来の象牙細管構造を有した修復象牙質の形成を促進する直接覆髄材料の開発が求められている。本研究結果で着目した低分子であるドーパミンは、安全かつ安価で、象牙細管構造を有する緻密な修復象牙質形成を誘導する、新規直接覆髄材料の開発につながると考えられる。