A Mechanobiological Approach to Elucidate the Control Mechanism of Zirconia Biocompatibility

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K17003
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57040:Regenerative dentistry and dental engineering-related
• Research Institution: Tohoku University
• Principal Investigator: SUN LU 東北大学, 歯学研究科, 助教 (10897606)
• Project Period (FY): 2021-04-01 – 2023-03-31
• Keywords: osteoblast / zirconia / cell adhesion

Outline of Final Research Achievements

We introduced two osteoblastic cell lines-- MC3T3-E1 and Saos-2 cells into this research. We seed the cells respectively on the control plate, CpTi, 3Y-TZP and NanoZr for 14 days. Then the cell proliferation ability and ALP ability was detected The result shows that both cell lines can attach and culture well on all materials and Saos2 cells show a better osteogenesis ability on zirconia and titanium than the control group. However, MC3T3-E1 cells could not conduct the osteogenesis ability well on all materials. Interestingly, in PCR tests of Saos-2 cells group, syndecan shows an increased expression on CpTi but a low expression on 3Y-TZP and NanoZr. The different expression levels of syndecans indicate that HSPGs might induce different mechanisms on titanium and zirconia. We pre-treate the cells with RDG (Arg-Fly-Asp-Ser) and nagstive control reagent RGE (Arg-Fly-Glu-Ser) and intend to detect the expression of syndecan family on different materials.

Free Research Field

Prosthodontics

Academic Significance and Societal Importance of the Research Achievements

Zirconia is a potential material for dental implants, but still exist shortcuts such as high toughness, low-temperature degradation and long-term stability. In this study, we intend to investigate the cell ability on zirconia, detect the mechanism behind, and aiming to improve zirconia materials.