Development of self-multilayered cell sheets for bone regeneration.

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K17029
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57040:Regenerative dentistry and dental engineering-related
• Research Institution: The Nippon Dental University
• Principal Investigator: Mochizuki Mai 日本歯科大学, 生命歯学部, 准教授 (90821934)
• Project Period (FY): 2021-04-01 – 2023-03-31
• Keywords: 歯髄幹細胞 / 無血清培養 / 自己多層化 / 細胞シート / 硬組織再生

Outline of Final Research Achievements

The aim of this study was to develop a novel cell sheet engineering method known as the “self-multilayered cell sheets” using dental pulp stem cells (DPSCs), for safe and efficient bone regeneration therapy. The self-multilayered cell sheets were synthesized by optimizing the serum-free culture conditions. The cell sheets had optimal strength and were easily manipulated by forceps. Moreover, they had the ability to form hard tissues in vitro and in vivo.
Our novel cell sheet engineered technology will contribute to the safe and effective hard tissue regenerative medicine using DPSCs.

Free Research Field

再生医療

Academic Significance and Societal Importance of the Research Achievements

社会的な再生医療に対する期待の高まりにともない、細胞を用いた再生医療は安全性と有効性が求められ、さらに治療技術の高い再現性を備えた医療提供が望まれる。
本研究は、異種血清を含まない無血清培養下で歯髄幹細胞（DPSC）を培養して、短期間で効率的に自己多層化細胞シートを形成する新技術を開発した（特許出願中）。さらに、優れた操作性と強度を有する自己多層化細胞シートは、高い硬組織形成能を示した。
本研究成果から、自己多層化細胞シートによる安全かつ効果的な硬組織再生医療が期待される。