Creation of biomaterials containing autologous crosslinked hyaluronic acid and growth factors and basic evaluation of their therapeutic effects

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K17070
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57050:Prosthodontics-related
• Research Institution: Iwate Medical University
• Principal Investigator: Hatakeyama Wataru 岩手医科大学, 歯学部, 研究員 (20733728)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 骨補填材料 / ヒアルロン酸 / インプラント

Outline of Final Research Achievements

A thiol-modified hyaluronic acid-based nHAP/BMP composite sample was prepared as an injectable sample. An EGDE-crosslinked hyaluronic acid/alkali-treated gelatin-based nHAP/BMP composite sample was also prepared as a freeze-dried implant sample. Various scientific and engineering evaluations were performed on both samples, and results were observed and measured that predicted an extension of the in vivo survival period and an improvement in the protein sustained release ability. Animal experiments were also performed on mouse and rat skulls, and bone regeneration from the set bone defect was observed for the freeze-dried implant sample, and vertical bone formation within the implant ring was observed for the implant sample. Significant bone formation was observed in the BMP-containing group compared to the control group. These results have been published in two English papers.

Free Research Field

歯科補綴学

Academic Significance and Societal Importance of the Research Achievements

従来、ヒアルロン酸は創傷治癒促進や関節の緩衝潤滑剤を中心とした用途で用いられてきた。本研究ではヒアルロン酸を基材として骨補填材としての検討を行い、新たに骨領域における骨増生用DDS材料として使用可能であることを見出した。この成果は、普及が進んでいヒアルロン酸ベース骨補填材の実用化に向けた大きな一助になると考えられる。また、注入型ヒアルロン酸骨補填材においては、注射針の刺入のみの低侵襲な方法で骨増生を達成する可能性を示唆しており、患者の身体的負担の軽減や術者の施術操作の簡易化などが期待できる。これらメリットは、現在の骨増生術に対するハードルを大きく引き下げる、大きな成果である。