Elucidating the supply and differentiation maturation kinetics of odontoblast induced by orthodontic force

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K17155
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57070:Developmental dentistry-related
• Research Institution: Niigata University
• Principal Investigator: Mizukoshi Masaru 新潟大学, 医歯学総合病院, 医員 (20882731)
• Project Period (FY): 2021-04-01 – 2023-03-31
• Keywords: 歯根膜 / 矯正的歯の移動 / 破歯細胞 / 歯根吸収

Outline of Final Research Achievements

This study aimed to elucidate the origin and differentiation/maturation process of odontoclast generated on the root surface by excessive orthodontic force by cell tracking method using light-converting fluorescent mice and by analyzing the expression of differentiation markers. The obtained fluorescence-expressing mice were heterozygous at the time of purchase, so they were bred to be homozygous to obtain stronger fluorescence intensity. We attempted to convert the fluorescent color of periodontal ligament cells by irradiating blue-violet light into the oral cavity of the mice. The cells on the surface near the light source were fully converted, but those near the apex of the root were insufficient. We are currently optimizing the irradiation conditions.

Free Research Field

細胞組織学

Academic Significance and Societal Importance of the Research Achievements

歯根吸収は矯正的歯の移動に際しておきる偶発症として代表的なもののひとつであり、発症メカニズムの解明と予防法の確立が急務である。歯根吸収を生じさせる破歯細胞は、破骨細胞と同様に造血幹細胞に由来すると考えられているが、細胞の由来と分化成熟についての基礎的情報は極めて乏しい。さらなる研究が必要であるが、破骨細胞と破歯細胞の類似性／相違点を明らかにすることは、歯根吸収を回避するための方法の探索へと繋がる。期待される研究成果は細胞生物学的エビデンスに基づく矯正歯科学の臨床術式の確立へとつながる研究である。