Comprehensive analysis of bacterial flora in orthodontic patients using next-generation sequencers and application to the establishment of risk management methods

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K17161
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57070:Developmental dentistry-related
• Research Institution: Hiroshima University
• Principal Investigator: Kado Isamu 広島大学, 医系科学研究科(歯), 助教 (50846847)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 矯正歯科治療 / 口腔衛生

Outline of Final Research Achievements

Our research team examines the oral cavity of patients about to start orthodontic treatment, we have continued to perform not only orthodontic problems but also PCR and caries risk tests using saliva, and oral bacterial flora analysis. We also analyzed the relationship between maxillofacial morphology and saliva properties, and found that the facial angle is closely related to saliva properties and the number of cariogenic bacteria. In particular, the amount of saliva secreted, its buffer capacity, and pH showed an inverse correlation with the facial angle. The number of cariogenic bacteria showed a positive correlation with ∠ANB and a negative correlation with the interincisal angle, so patients with maxillary protrusion and labial inclination of the anterior teeth, such as those with Angle Class II, devision 1, are considered to be at high risk for caries. We have presented these results at domestic conferences and published papers in international journals.

Free Research Field

矯正歯科学

Academic Significance and Societal Importance of the Research Achievements

現在、矯正歯科治療は一般的な歯科治療の一部となり、治療を受ける人口も増加している。しかし、その治療過程ではう蝕や歯肉炎などの医原性障害が発生することがあるため、これらの予防策を立案することが急務である。その観点から、我々は口腔衛生状態と細菌叢との関連性について研究を重ねてきた。今後、この研究を継続していくことで、矯正歯科治療中に発生する医原性障害を予防するシステムを構築していくことが可能になると思われる。そこに到達する上でのデータの蓄積および解析ができたという点で、本研究は大きな社会的意義を有していると思われる。