Establishment of minimally invasive bone regeneration for cleft lip and palate using mesenchymal stem cell exosomes derived from deciduous dental pulp

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K17186
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57070:Developmental dentistry-related
• Research Institution: Hiroshima University
• Principal Investigator: Abe Takaharu 広島大学, 病院(歯), 助教 (20806682)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: エクソソーム / 培養上清 / SHED / DPSC / HCEM / 骨再生 / miRNA

Outline of Final Research Achievements

Exosomes extracted from the culture supernatant of mesenchymal stem cells (MSCs) isolated and cultured from the dental pulp of deciduous teeth were called SHED-EXs, and exosomes isolated from the culture supernatant of MSCs isolated from the pulp of permanent teeth were called DPSC-EXs. It was used for. An increase in cell number was observed by adding SHED-Exo and DPSC-EXs to bone marrow-derived mesenchymal stem cells (BMSCs) and human osteoblasts (HCO). Furthermore, when SHED-EXs and DPSC-EXs were added to BMSCs and HCOs that had been induced to undergo osteogenic differentiation, and quantitative evaluation was performed using alizarin red staining, it was found that the addition of exosomes enhanced osteogenic differentiation.

Free Research Field

矯正歯科

Academic Significance and Societal Importance of the Research Achievements

脱落あるいは便宜的に抜去する乳歯あるいは永久歯より、採取される歯髄は、従来行われてきた腸骨より採取する骨髄を用いるよりも低侵襲である。また、その培養上清より抽出した、エクソソームの骨形成への関与について解明した点において、本研究の学術的意義は高い。miRNAおよび細胞増殖、ALPやMAPKを介して、骨形成を促進させることを明らかとした。今後さらに、エクソソームを臨床応用し患者の負担が軽減され、低侵襲で良好な骨再生療法を確立する、社会的観点から重要な意義がある。