Molecular mechanisms that define muscle hypertrophy and injury set points during skeletal muscle eccentric contraction.

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K17589
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 59020:Sports sciences-related
• Research Institution: Juntendo University
• Principal Investigator: Nakada Satoshi 順天堂大学, 大学院スポーツ健康科学研究科, 特任助教 (20778881)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 骨格筋 / 細胞損傷

Outline of Final Research Achievements

In this study, a mechanical stress loading model was constructed using myotubular cells derived from iPS cells of patients with muscle diseases, and cell damage was evaluated. The activity of muscle deleterious enzymes leaked into the culture supernatant 24 hours after extension stimulation was significantly higher in the diseased strain than in the repaired strain. In addition, miRNAs leaked into the culture supernatant were increased by stretch stimulation in the diseased strain. The expression level of sarcomere damage repair genes after 24 hours of extension stimulation was significantly higher in the disease strain than in the repair strain. This indicates that muscle aberrant enzyme and miRNA leakage are more likely to occur upon stretch stimulation in the diseased strain and that sarcomere damage occurs more intensely in this strain.

Free Research Field

骨格筋生理学

Academic Significance and Societal Importance of the Research Achievements

本研究では、iPS細胞から分化した筋管細胞を用いた新たな細胞培養系を構築し、機械的伸展刺激装置によるメカニカルストレスを負荷する実験系を構築した。
このモデルから得られた知見により、筋細胞が損傷をした際のバイオマーカーとなりうる因子が見つかったことから、細胞培養系だけにとどまらず、アスリートを含めたヒトを対象とする筋損傷発生のリスク予測研究に応用することができると考えられる。