Elucidation of isotope labeled DHA and EPA hydroperoxide distribution and metabolism

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K17678
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 59040:Nutrition science and health science-related
• Research Institution: Akita University
• Principal Investigator: Yoshinaga-Kiriake Aya (桐明絢) 秋田大学, 理工学研究科, 助教 (20794845)
• Project Period (FY): 2021-04-01 – 2023-03-31
• Keywords: 安定同位体ラベル化脂肪酸 / 過酸化脂質 / EPA過酸化物 / 呼気 / 体内動態 / 体燃焼性 / 同位体比質量分析計

Outline of Final Research Achievements

To elucidate the effects of ingestion of lipid hydroperoxides in human body, the catabolic rates of synthesized 13C-labeled EPA ethyl ester hydroperoxides (*EPA-EE-OOH) and EPA ethyl ester (*EPA-EE) in the mouse were compared using isotope ratio mass spectrometry. Although the catabolic rate of *EPA-EE-OOH was faster than that of *EPA-EE in the mouse, there was no significant differences in total amount of catabolism among *EPA-EE-OOH and *EPA-EE. In addition, it has been shown that EPA ethyl ester hydroperoxides were decomposed to fatty acid and oxo-fatty acid and aldehyde by the treatment of hydrogen chloride. Thereafter, these decomposition products were β-oxidized rapidly in the liver.

Free Research Field

油脂科学

Academic Significance and Societal Importance of the Research Achievements

本研究結果により、EPA過酸化物の体内動態の一部を明らかにすることができ、EPA過酸化物は胃酸で分解され、EPAより分子量の小さい脂肪酸やアルデヒド類に変換されることが判明した。本研究のように、多価不飽和脂肪酸過酸化物の体内動態を解明する一連の研究が推進されれば、どの脂質過酸化物がヒトの健康に悪影響を及ぼすのかを解明する糸口となる。さらに、悪影響を及ぼす脂質過酸化物を除去した食品や体に良い脂質の開発にも応用可能であり、学術的な波及効果だけでなく、社会的な波及効果も極めて高いと考える。