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2023 Fiscal Year Final Research Report

Methane Fermentation of Persistent Biomass Based on Elucidation of Rumen Microbial Symbiosis Mechanism

Research Project

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Project/Area Number 21K17909
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 64030:Environmental materials and recycle technology-related
Research InstitutionIshikawa Prefectural University

Principal Investigator

Yasunori Baba  石川県立大学, 生物資源環境学部, 講師 (70747823)

Project Period (FY) 2021-04-01 – 2024-03-31
Keywordsルーメン微生物 / リグノセルロース / 酵素活性 / リアルタイムPCR / RNA-seq
Outline of Final Research Achievements

We have improved the efficiency of methane fermentation by using lignocellulolytic bacteria in bovine rumen fluid to solubilize plant biomass, which is then used for methane fermentation. However, lignocellulolytic bacteria in rumen fluid significantly reduce lignocellulose degradation once they are released outside the cattle body. In order to put the methane fermentation method using rumen microbes into practical use, it is necessary to establish conditions under which lignocellulolytic rumen microbes can maintain growth and enzyme production even in an environment outside the cattle body (inside the reactor). We have optimized the culture conditions and successfully produced cellulases in the reactor for more than one year.

Free Research Field

環境微生物

Academic Significance and Societal Importance of the Research Achievements

2013年に私たちが論文投稿(Bioresource Technology 128. 94-99)して以来,世界中で牛ルーメン微生物を用いたメタン発酵前処理に関する研究が行われ始めた。しかし,これまでに,ルーメン微生物の連続培養に成功し,実用化した事例は私たちの知る限りない。本研究は,一年以上にわたり,セルラーゼ活性を維持したままルーメン微生物の連続培養に成功した。さらに,パイロットプラントを,スーパーマーケットに設置し,世界ではじめて実証試験を開始した。本研究は,これまでメタン発酵困難であった植物系バイオマスを利用可能にするとともに,震災時には雑草発電により地域のエネルギー自給にも資する。

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Published: 2025-01-30  

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