2023 Fiscal Year Final Research Report
Methane Fermentation of Persistent Biomass Based on Elucidation of Rumen Microbial Symbiosis Mechanism
Project/Area Number |
21K17909
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 64030:Environmental materials and recycle technology-related
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Research Institution | Ishikawa Prefectural University |
Principal Investigator |
Yasunori Baba 石川県立大学, 生物資源環境学部, 講師 (70747823)
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Project Period (FY) |
2021-04-01 – 2024-03-31
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Keywords | ルーメン微生物 / リグノセルロース / 酵素活性 / リアルタイムPCR / RNA-seq |
Outline of Final Research Achievements |
We have improved the efficiency of methane fermentation by using lignocellulolytic bacteria in bovine rumen fluid to solubilize plant biomass, which is then used for methane fermentation. However, lignocellulolytic bacteria in rumen fluid significantly reduce lignocellulose degradation once they are released outside the cattle body. In order to put the methane fermentation method using rumen microbes into practical use, it is necessary to establish conditions under which lignocellulolytic rumen microbes can maintain growth and enzyme production even in an environment outside the cattle body (inside the reactor). We have optimized the culture conditions and successfully produced cellulases in the reactor for more than one year.
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Free Research Field |
環境微生物
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Academic Significance and Societal Importance of the Research Achievements |
2013年に私たちが論文投稿(Bioresource Technology 128. 94-99)して以来,世界中で牛ルーメン微生物を用いたメタン発酵前処理に関する研究が行われ始めた。しかし,これまでに,ルーメン微生物の連続培養に成功し,実用化した事例は私たちの知る限りない。本研究は,一年以上にわたり,セルラーゼ活性を維持したままルーメン微生物の連続培養に成功した。さらに,パイロットプラントを,スーパーマーケットに設置し,世界ではじめて実証試験を開始した。本研究は,これまでメタン発酵困難であった植物系バイオマスを利用可能にするとともに,震災時には雑草発電により地域のエネルギー自給にも資する。
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