2021 Fiscal Year Research-status Report
Construction of a multi-organ interaction model via the controlled vascularisation of modular organoids
| Project/Area Number |
21K18048
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Koh IsabelSiewYin 国立研究開発法人理化学研究所, 開拓研究本部, 特別研究員 (90868415)
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| Project Period (FY) |
2021-04-01 – 2023-03-31
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| Keywords | Cube device / Multi-directional / Vascularisation |
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| Outline of Annual Research Achievements |
The objective was to create a precisely-positioned blood vascularisation channel in a modular cube culture device. 3D printing of carbohydrate glass was used to create a water-dissolvable helix-shaped mould that was then placed in hydrogel in the cube. Once the gel was cured, the mould was removed by immersing in phosphate buffer solution (PBS), leaving behind a mould-shaped pocket in the gel. Endothelial cells were then seeded in the helix-shaped channels that remained in the gel to create a vascular channel. Imaging of the vascular channel was achieved by taking images from multiple directions of the cube and combining the images together. This achievement demonstrated the advantage of the cube device in precisely controlling the positioning of cells in 3D culture, and multi-directional imaging. The development of this protocol opens up possibilities to generate complex organoids in the cube device.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
The achievement to control the positioning of cells in the cube, and establishment of method to precise create a channel in the cube by punching is currently being applied to other organs to create organoids with more complex structure and a mix of different cell types.
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| Strategy for Future Research Activity |
With the current method developed, the channel sizes are approximately 1 mm in diameter, which is too large for most applications. In the future, other methods for creating smaller diameter channels are being explored.
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| Causes of Carryover |
Amount too small to buy anything useful
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Research Products
(4 results)
