2022 Fiscal Year Annual Research Report
Construction of a multi-organ interaction model via the controlled vascularisation of modular organoids
| Project/Area Number |
21K18048
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Koh IsabelSiewYin 国立研究開発法人理化学研究所, 開拓研究本部, 特別研究員 (90868415)
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| Project Period (FY) |
2021-04-01 – 2023-03-31
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| Keywords | Organ-on-a-chip / Tissue interaction / 3D culture / Blood-brain barrier / Organ-organ interaction |
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| Outline of Annual Research Achievements |
The aim of this research was to mimic tissue-tissue interactions by combining in a chip device tissues separately cultured in a CUBE device. This was demonstrated with a blood-brain barrier (BBB)-brain model. The BBB module was created by seeding astrocytes and pericytes in Matrigel in the CUBE, with iPSC-derived brain microvascular endothelial cells (BMECs) seeded on the top surface of the gel. The brain module consisted of brain cancer cells encapsulated in Matrigel in the CUBE. Both BBB and brain modules were assembled in a chip device, and a drug for treating brain cancer was applied to the BBB side to simulate the testing of a drug's permeability into the brain. The proof-of-concept showed that tissue-tissue interaction can be mimicked in vitro using the platform developed here.
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