Bioactive gas pump-probe method elucidates the relationship of nitric oxide diffusion with vascular function

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K18192
• Research Category: Grant-in-Aid for Challenging Research (Pioneering)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 28:Nano/micro science and related fields
• Research Institution: Kyoto University
• Principal Investigator: Furukawa Shuhei 京都大学, 高等研究院, 教授 (90452276)
• Co-Investigator(Kenkyū-buntansha): 猪瀬 朋子 京都大学, 白眉センター, 特定准教授 (10772296) ; 亀井 謙一郎 京都大学, 高等研究院, 研究員 (00588262) ; 雲林院 宏 北海道大学, 電子科学研究所, 教授 (40519352)
• Project Period (FY): 2021-07-09 – 2024-03-31
• Keywords: 一酸化窒素 / 多孔性材料 / ナノマテリアル

Outline of Final Research Achievements

The aim of this study was to develop fundamental technology for the "biological gas pump-probe method," which synchronizes NO production and detection within cells. Specifically, we constructed a system that combines intracellular endoscopy technology using metal nanowires with NO-releasing metal-organic frameworks (MOFs). Using one-dimensional plasmonic metal nanowires with a diameter of approximately 100 nm, we enabled remote excitation of surface-enhanced Raman scattering (RE-SERS) and applied this waveguide for remote molecular release within cells. By utilizing a light-responsive NO-releasing MOF (named NOF-1), we developed a technology for remotely releasing NO at specific locations within cells. We successfully performed NO release within human smooth muscle cells and detected NO using a fluorescence probe.

Free Research Field

材料化学

Academic Significance and Societal Importance of the Research Achievements

生体内で産生されるNOは、血管弛緩・拡張を惹起することが知られており、その効果を活用した治療薬への応用が期待されている。本研究では平滑筋細胞内へのNO放出と検出に成功した。今後NOをより汎用性の高い治療薬として活用するため、本技術を用いて治療部位でのNOガス拡散の時空間的挙動と血管機能の相関を解明し、必要最低限のNOの利用へとつなげることが可能になる。