2022 Fiscal Year Final Research Report
Development of cell-cell tensile strength measurement technique to elucidate cell differentiation mechanisms involved in cancer metastasis
| Project/Area Number |
21K18672
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 18:Mechanics of materials, production engineering, design engineering, and related fields
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| Research Institution | Shizuoka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
大多 哲史 静岡大学, 工学部, 准教授 (30774749)
倉科 佑太 東京農工大学, 工学(系)研究科(研究院), 准教授 (40801535)
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| Project Period (FY) |
2021-07-09 – 2023-03-31
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| Keywords | 細胞接着 / 磁気工学 / マイクロデバイス / 材料強度学 |
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| Outline of Final Research Achievements |
In this study, we established a scheme for measuring cell-cell tensile strength to elucidate the cell differentiation mechanism involved in cancer metastasis. In order to measure cell-cell adhesion strength, it is necessary to prepare two cell-guided objects at the tip of a measurement substrate and connect them to form cell-cell adhesion at the tip of the substrate. Thus, we focused on single cell guidance behavior and achieved optimization of a substrate geometry that can guide cells with magnetic nanoparticles incorporated by a magnetic field generated by a magnet.
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| Free Research Field |
磁気工学
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| Academic Significance and Societal Importance of the Research Achievements |
細胞特性は細胞接着性と密接に関係しているものの，細胞-細胞間の接着強度をダイレクトに測定する手法は存在せず，ある基板に接着した細胞の強度を擬似的に計測するに留まっている．一方で本研究では，“磁力で誘導した細胞を押さえつけて引っ張る”という新発想を具現化し，接着細胞-細胞間の引張強度計測スキームを構築した．本研究で明らかにした単一細胞誘導に適した基板形状パラメータをもとに，細胞間接着強度計測への展開が期待される．
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