2022 Fiscal Year Final Research Report
Development of a single-cell thermal analysis platform with sub-nW resolution
Project/Area Number |
21K18694
|
Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
|
Allocation Type | Multi-year Fund |
Review Section |
Medium-sized Section 19:Fluid engineering, thermal engineering, and related fields
|
Research Institution | Kyushu Institute of Technology |
Principal Investigator |
Yabuki Tomohide 九州工業大学, 大学院工学研究院, 准教授 (70734143)
|
Project Period (FY) |
2021-07-09 – 2023-03-31
|
Keywords | バイオカロリメトリ / ナノカロリメータ / 単一細胞 |
Outline of Final Research Achievements |
We developed a highly sensitive calorimeter (freestanding microchannel type nanocalorimeter) with extremely high thermal resistance on the order of 10^6 K/W. The developed nanocalorimeter can be fabricated by two simple processes without using costly MEMS fabrication techniques: heat-drawing a glass capillary to form the microchannel part and depositing a chromium nitride thin film thermistor on the side surface of the microchannel. Using the nanocalorimeter installed inside a self-built vacuum thermostatic chamber, we succeeded in measuring the metabolic heat of a single paramecium. We also demonstrated that the nano-DTA using the developed nanocalorimeter can accurately measure phase transition heat at the sub-micro joule level.
|
Free Research Field |
熱工学
|
Academic Significance and Societal Importance of the Research Achievements |
細胞状態の診断や細胞機能の理解のために,単一細胞熱分析が一つの有効な技術と考えられる中で,本研究を通じて,単一細胞の代謝熱を計測できるセンサ構造,計測システムを構築できたことは意義深い.今後,センサのさらなる高感度化により,個体差や状態変化を熱的に検出できる技術の開発を目指す.
|