2022 Fiscal Year Final Research Report
Study of polarization of dielectric particles for developing rapid diagnosing methods
Project/Area Number |
21K18728
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Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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Allocation Type | Multi-year Fund |
Review Section |
Medium-sized Section 21:Electrical and electronic engineering and related fields
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Research Institution | Kyushu University |
Principal Investigator |
Nakano Michihiko 九州大学, システム情報科学研究院, 准教授 (00447856)
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Project Period (FY) |
2021-07-09 – 2023-03-31
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Keywords | 微粒子 / 分極 / 屈折率 / 誘電率 / DNA分子 / エクソソーム |
Outline of Final Research Achievements |
This study aimed to investigate the polarization of a dielectric particle by considering its surface complex permittivity consisting of electric conductivity and permittivity. The surface conductivity can be estimated by measuring its zeta potential. Otherwise, a method to measure the surface permittivity has not been established. This study experimented on whether the laser diffraction from it represents the difference in its surface permittivity. As a result, DNA-labeled and non-labeled microbeads showed different diffraction patterns. It could mean the difference in the permittivity of the DNA attached to the particle surface. The dielectric property of exosomes was investigated. The exosome is one of the extracellular vesicles and is considered a biomarker for cancer diagnosis. From the measured dielectrophoretic property of the exosomes derived human breast cancer and normal cells, the membrane capacitance of the exosomes represents almost the same as that of the derived cells.
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Free Research Field |
静電気生物応用
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Academic Significance and Societal Importance of the Research Achievements |
本研究では、様々なバイオ関連物質を検出するための共通のプラットフォーム構築を目指し,微粒子表面に付着した物質の誘電率を測定する方法を考案した.これまでにない新しい手法として,微粒子のレーザー光の回折・散乱光を用いることを試みた.その結果,表面に結合DNA分子によって,回折・散乱光が変化することを示し,本手法がこれまでは不可能であった微粒子表面の誘電率測定の手法として使用できる可能性を示した.また,エクソソームの誘電体特性が由来細胞によって異なることを示し,特に,がん細胞由来エクソソームの特性を明らかにし,エクソソームを用いた診断の簡素化・迅速化の可能性を示した.
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