2023 Fiscal Year Final Research Report
Functional expression system for plant ion transport system in Escherichia. coli
| Project/Area Number |
21K19060
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 38:Agricultural chemistry and related fields
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2021-07-09 – 2024-03-31
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| Keywords | イオン輸送体 / Kチャネル / Arabidopsis thaliana / E. coli / 輸送解析 |
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| Outline of Final Research Achievements |
We have pursued investigations aimed at elucidating the functional expression of homologs of uncharacterized plant potassium transporters. Based on reports of expression of eukaryotic ion transporters in Escherichia coli, we created multiple mutants of potassium and sodium transporters in E. coli, followed by elucidating their transport activities and physiological roles, thereby revealing the function and roles of homologous ion transporters in eukaryotes. Furthermore, successful measurement of potassium transport activity was achieved for previously uncharacterized plant potassium channels using heterologous expression systems. Analysis was conducted on the expression tissues and roles of potassium channels. As a result, the potassium channel activity of functionally unreported plant ion transport system was detected, clarifying their functional expression within plants.
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| Free Research Field |
膜輸送機能学
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| Academic Significance and Societal Importance of the Research Achievements |
機能未同定の植物イオン輸送体のＫチャネル活性が明らかになり，植物内における養分吸収や恒常性維持に関与する分子の一つが明らかになった．この結果は，他の生物において機能未同定のイオン輸送体の機能解明の可能性を示唆する．本申請で扱ったKチャネルは動物，微生物やウイルスにも存在することから，同様の測定手段を踏襲することで新たにイオン輸送体の解明が可能となる．また，本研究で明らかになった大腸菌のK輸送体のホモログは真核生物にも存在しており，そのK輸送体の機能と役割に関する情報となる．
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