Development of a simple anaerobic microbial culture system utilizing the functions of microorganisms

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K19075
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 38:Agricultural chemistry and related fields
• Research Institution: Kyoto University
• Principal Investigator: Kishino Shigenobu 京都大学, 農学研究科, 准教授 (40432348)
• Project Period (FY): 2021-07-09 – 2024-03-31
• Keywords: 腸内細菌 / 嫌気性微生物 / 嫌気反応 / 嫌気

Outline of Final Research Achievements

In recent years, interest in gut bacteria has been increasing. However, most research in the field of applied microbiology is conducted under "aerobic conditions" where oxygen is present. There is little research done under "anaerobic conditions," where gut bacteria actually exist and oxygen is absent.
As a result, researchers attempting to study gut bacteria face significant barriers, such as the need to introduce equipment that can create "anaerobic conditions." Therefore, this study focuses on developing a system that can easily create "anaerobic conditions."

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

腸内細菌などの嫌気性微生物を扱う研究は、設備投資がいること、嫌気性を維持するためのスキルが必要であること、などの理由からあまり進んでいない。本研究成果では、研究者が容易に扱うことができる大腸菌を用いた形質転換体を用いることで、好気性細菌を扱うかの如く嫌気性微生物を扱うことが可能となることから、嫌気性微生物に関する研究分野が飛躍的に発展する可能性を秘めている。