2022 Fiscal Year Final Research Report
Study of the molecular mechanisms involved in regulation of meristem tissue in potato tuber
| Project/Area Number |
21K19102
|
|---|
| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Medium-sized Section 39:Agricultural and environmental biology and related fields
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
藤野 介延 北海道大学, 農学研究院, 教授 (80229020)
松下 陽介 国立研究開発法人農業・食品産業技術総合研究機構, 植物防疫研究部門, チーム長 (00414665)
|
|---|
| Project Period (FY) |
2021-07-09 – 2023-03-31
|
| Keywords | ジャガイモ / 塊茎 / 二次成長 / ウイロイド |
|---|
| Outline of Final Research Achievements |
The irregular shape of potato tubers is caused by secondary growth during tuber development, and the molecular mechanisms involved in bud elongation and dormancy during tuber development are still unclear. In this study, we aimed to elucidate the molecular mechanisms involved in sprouting and dormancy of tubers, and analyzed the effects of plant hormones on sprouting of tubers using microtubers. RNA-seq analysis suggested that cis-zeatin, whose function is not well known as cytokinin in various plants, is involved in microtuber sprouting. Chrysanthemum stunt viroid (CSVd) is known to disturb dormancy state of potato tubers. We inoculated CSVd to potato plants and then the obtained shoots were cultured in vitro to establish potato cultures retaining CSVd infection. By culturing the CSVd-infected potato cultures at different conditions with plant hormone, we will further investigate in detail the effects of CSVd infection on the development and germination of microtubers.
|
| Free Research Field |
植物生理学、植物病理学
|
| Academic Significance and Societal Importance of the Research Achievements |
食用部位である塊茎の発達や休眠を制御するメカニズムはジャガイモの生理学的研究で最も重要である。ジャガイモは栄養繁殖性であり、倍数性遺伝子構造をもつために表現型への影響が大きい遺伝子変異体を得るのは容易でない。本研究ではウイロイド感染が塊茎形成時に特異的に形態異常を引き起こすことに着目し、この病原体の病徴メカニズムを解析するための実験系を確立した。ウイロイドによる病徴誘導メカニズムの詳細を明らかにすることは塊茎発達や萌芽に関わる分子機構を解明することにもなり、ジャガイモ有用品種の分子育種や困難とされているウイロイド病防除へも役立つ知見となるものである。
|
